Over the years since Equestria’s birth, some ponies have had relationships with other creatures, therefore producing hybrids which many were afraid of. Persecution rose and many of the hybrids and their parents feared for their lives. Thankfully the culling and hunts have ended over time. However many still hold prejudice against ‘dirty bloods’.
The Mane Six
Princess Twilight Sparkle: Species: Alicorn. Age: 35. Gender: Female. Bio: Much hasn’t changed for the new Princess (canon back story wise). She has done her duties faithfully and is a treasure to all. However she has noticed some of the mistreatment the hybrid ponies have gone through. Having two friends who are hybrids, she is looking more into this conflict.
Rarity: Species: Unicorn. Age: 34. Gender: Female. Bio: Rarity still owns the boutique down in Ponyville (her canon back story hasn’t changed). She’s currently dating Spike (who is 22). Rarity is also helping Twilight in her investigations